GlycoErase™ AMY2B Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF500)

Description	GlycoErase™ AMY2B knockout HEK293T cell line is a human embryonic kidney (HEK293T) model lacking AMY2B expression. AMY2B encodes a pancreatic alpha-amylase isoform that catalyzes early steps of dietary polysaccharide degradation. Disruption in HEK293T cells provides a human context to interrogate amylase isoform diversity, transcriptional and post-translational regulation of secreted glycosidases, and nutrient-responsive signaling, with uses in carbohydrate metabolism research and biocatalyst development.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	AMY2B
Full Name	Amylase Alpha 2B
Alternative Name	HXA; AMY2; AMY3
Location	1p21.1
Gene ID	280
Summary	Pancreatic alpha-amylase, an enzyme involved in starch and glycogen digestion, hydrolyzes 1,4-alpha-glucoside bonds. It belongs to a gene cluster expressed in the pancreas.