GlycoErase™ AXIN1 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF618)

Description	GlycoErase™ AXIN1 knockout HepG2 cell line lacks AXIN1, a scaffold protein regulating Wnt/β-catenin signaling through interactions with APC, GSK3β, and β-catenin, and also involved in apoptosis regulation. AXIN1 mutations are linked to cancers including hepatocellular carcinoma. This knockout provides a human liver model to study Wnt pathway regulation, tumorigenesis, and apoptotic control, supporting oncology research and therapeutic development.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	AXIN1
Full Name	Axin 1
Alternative Name	AXIN; CMDOH; PPP1R49
Location	16p13.3
Gene ID	8312
Summary	This cytoplasmic protein contains RGS and DIX domains and binds APC, beta-catenin, glycogen synthase kinase 3 beta, and protein phosphatase 2. It suppresses WNT pathway signaling and can trigger apoptosis. Mutations are linked to various cancers; multiple isoforms exist.