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GlycoErase™ B3GLCT Knockout CHO-K1 Cell Line

CAT#: GLJF-0425-JF115

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Overview Specification Target Information Related Products
Overview
Description
The B3GLCT knockout (CHO-K1) cell line, without B3GLCT beta 3-glucosyltransferase, may disrupt O-linked glycosylation of TSRs in CHO cells and potentially contribute to Peters-plus syndrome-related studies, B3GLCT transfers glucose to O-linked fucosylglycans on thrombospondin type-1 repeats, relevant to O-glycan modification of TSR-containing therapeutic proteins in CHO.
Product Type
KO Cell Lines
Species
Hamster
Cell Morphology
Epithelial, adherent
Passage Ratio
1:3~1:5
Cell Line
CHO-K1
Lineage
Chinese hamster ovary
Specification
Cell Viability
>90%
Sterility Test
The sterility test indicated an absence of microbial growth.
Identity Test
STR identification
Mycoplasma Test
Negative
Virus Test
Negative for HIV, HBV and HCV.
Genetic Stability Testing
We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation
PCR, Sanger Sequencing
Culture Medium
FBS & Penicillin/Streptomycin & Proline & RPMI
Application
Functional assay
Size
1 M cells/vial*2
Product Format
Frozen
Shipping
Dry ice
Availability Status
Made to order
Handling Notes
Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer
This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.
Target Information
Target
B3GLCT
Full Name
Beta 3-glucosyltransferase
Alternative Name
B3Glc-T, B3GTL, B3GALTL
Location
13q12.3
Gene ID
145173
Summary
Beta-1,3-glucosyltransferase that transfers glucose to O-linked fucosylglycans on thrombospondin type-1 repeats; defects cause Peters-plus syndrome.
For Research Use Only.
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