GlycoErase™ B3GNT8 Knockout IST-Mes1 Cell Line (CAT#: GLJF-0425-JF888)

Description	The B3GNT8 knockout IST-Mes1 cell line lacks B3GNT8 protein, UDP-GlcNAc:betaGal beta-1,3-O-acetylglucosaminyltransferase 8 involved in poly-N-acetyllactosamine biosynthesis in exosomes. B3GNT8 knockout in IST-Mes1 cells enables study of exosomal poly-N-acetyllactosamine disruption in Pleural Mesothelioma, potentially affecting exosome glycosylation, exosome-mediated signaling and exosomal glycan functions in the tumor microenvironment.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:8
Cell Line	IST-Mes1
Primary Disease	Pleural mesothelioma
Lineage	Pleura
Lineage Subtype	Pleural mesothelioma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	B3GNT8
Full Name	UDP-GlcNAc:BetaGal Beta-1,3-O-acetylglucosaminyltransferase 8
Alternative Name	beta3Gn-T8, B3GALT7, BGALT15
Location	19q13.2
Gene ID	374907
Summary	Protein O-acetylglucosaminyltransferase involved in poly-N-acetyllactosamine biosynthesis; located in extracellular exosomes.