GlycoErase™ C1GALT1 Knockout Human Bladder Cancer Cell 5637 (CAT#: GLJF-0525-JF7)

Description	A human bladder bancer bell 5637 line engineered to lack C1GALT1 presents a distinctive in vitro system for the investigation of Core 1 O-glycosylation in bladder cancer; the C1GALT1 deficiency impairs the synthesis of Core 1 O-glycans, enabling researchers to analyze the impact of altered O-glycosylation profiles on the tumor's surrounding environment and its capacity to metastasize, as well as to pinpoint O-glycosylatioN-related therapeutic targets and drug candidates.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:3~1:6
Cell Line	5637
Primary Disease	Bladder Urothelial Carcinoma
Lineage	Bladder/Urinary Tract

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	C1GALT1
Full Name	Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-Beta-galactosyltransferase 1
Alternative Name	C1GALT, T-synthase
Location	7p22.1-p21.3
Gene ID	56913
Summary	Synthesizes core 1 O-glycan (Gal-beta-1-3GalNAc-R) via Gal transfer (UDP-Gal to GalNAc-alpha-1-R); precursor for mucin-type O-glycans (cell surface & secreted) involved in cell signaling and interactions.