GlycoErase™ C1GALT1 Knockout HepG2 Cell Line

The C1GALT1 knockout HepG2 cell line offers a unique platform for exploring O-glycosylation (Core 1) in liver cancer and its interaction with the hepatic environment. C1GALT1 deficiency impedes Core 1 O-glycan synthesis in HepG2 cells, potentially altering the glycosylation of mucin-like proteins expressed by liver cells. Researchers can analyze the effects of altered O-glycosylation on the tumor microenvironment in the liver, metastasis within the liver, and identify O-glycosylation targets or drugs specific to liver malignancies.

KO Cell Lines

Human

Epithelial-like, adherent

1:4~1:6

HepG2

Hepatoblastoma

Liver

Hepatoblastoma

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Glutamine

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

C1GALT1

Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-Beta-galactosyltransferase 1

C1GALT, T-synthase

7p22.1-p21.3

Synthesizes core 1 O-glycan (Gal-beta-1-3GalNAc-R) via Gal transfer (UDP-Gal to GalNAc-alpha-1-R); precursor for mucin-type O-glycans (cell surface & secreted) involved in cell signaling and interactions.