GlycoErase™ ENO1 Knockout CHO-K1 Cell Line (CAT#: GLJF-0825-JF273)

Description	GlycoErase™ ENO1 knockout CHO-K1 cell line is a CHO-K1 model in which ENO1 expression has been eliminated. ENO1 encodes alpha-enolase, a glycolytic enzyme also functioning as tau-crystallin in the lens and as a transcriptional regulator interacting with the c-Myc promoter. It has been identified as an autoantigen in Hashimoto encephalopathy. The knockout of ENO1 in the CHO-K1 background offers a tool to study glycolysis, autoimmunity, and tumor suppressor regulation, while supporting applications in cancer metabolism, autoimmune disease research, and translational therapeutics.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ENO1
Full Name	Enolase 1
Alternative Name	NNE; PPH; MPB1; ENO1L1; ENO1-IT1; HEL-S-17
Location	1p36.23
Gene ID	2023
Summary	This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.