GlycoErase™ ENO3 Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF506)

Description	GlycoErase™ ENO3 knockout HEK293T cell line is a human embryonic kidney (HEK293T) model lacking ENO3 expression. ENO3 is the muscle-biased enolase isoform involved in skeletal muscle energy metabolism and regeneration. Its removal in HEK293T cells offers a human platform to parse isoform compensation among enolases, evaluate glycolytic control nodes outside muscle tissue, and model variants linked to glycogen storage and myopathic phenotypes.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ENO3
Full Name	Enolase 3
Alternative Name	MSE; GSD13
Location	17p13.2
Gene ID	2027
Summary	Beta-enolase, found in adult skeletal muscle, plays a role in muscle development and regeneration. A switch from alpha-enolase to beta-enolase occurs during muscle development. Mutations in this gene are associated with glycogen storage disease.