GlycoErase™ EOGT Knockout KD Cell Line (CAT#: GLJF-0425-JF653)

Description	The EDGT knockout KD cell line, derived from an extra-cranial rhabdoid tumor, lacks EDGT protein, an EGF domain-specific O-GlcNAc transferase. EDGT mediates O-GlcNAc modification of extracellular EGF domain proteins. In rhabdoid cancer cells, EDGT knockout can elucidate O-GlcNAc's role in EGF signaling relevant to this pediatric sarcoma, cell-cell interactions, and microenvironment modulation via altered EGF domain protein glycosylation.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	KD cell line
Primary Disease	Rhabdoid cancer
Lineage	Soft Tissue
Lineage Subtype	Rhabdoid cancer

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	F-12 & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	EOGT
Full Name	EGF domain specific O-linked N-acetylglucosamine transferase
Alternative Name	C3orf64, AER61, FLJ33770
Location	3p14.1
Gene ID	285203
Summary	Catalyzes the transfer of N-acetylglucosamine to serine/threonine residues of proteins destined for the extracellular environment, a key step in N-linked glycosylation.