GlycoErase™ EPM2A Knockout CHO-K1 Cell Line (CAT#: GLJF-0825-JF275)

Description	GlycoErase™ EPM2A knockout CHO-K1 cell line is a CHO-K1 model in which EPM2A expression has been deleted. EPM2A encodes a dual-specificity phosphatase involved in preventing glycogen hyperphosphorylation and insoluble aggregate formation. Mutations in EPM2A are associated with Lafora disease, a fatal neurodegenerative disorder characterized by glycogen-derived inclusions. This knockout CHO-K1 model provides a research tool to investigate glycogen regulation, Lafora body formation, and neurodegenerative disease mechanisms, supporting applications in glycogen metabolism research, rare disease modeling, and neurobiology.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	EPM2A
Full Name	Epm2A Glucan Phosphatase, Laforin
Alternative Name	EPM2; MELF; MELF2
Location	6q24.3
Gene ID	7957
Summary	This gene encodes a dual-specificity phosphatase and may be involved in the regulation of glycogen metabolism. The protein acts on complex carbohydrates to prevent glycogen hyperphosphorylation, thus avoiding the formation of insoluble aggregates. Loss-of-function mutations in this gene have been associated with Lafora disease, a rare, adult-onset recessive neurodegenerative disease, which results in myoclonus epilepsy and usually results in death several years after the onset of symptoms. The disease is characterized by the accumulation of insoluble particles called Lafora bodies, which are derived from glycogen.