GlycoErase™ G6PC1 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF625)

Description	GlycoErase™ G6PC1 knockout HepG2 cell line is a human liver-derived HepG2 model lacking G6PC1, which encodes the catalytic subunit of glucose-6-phosphatase. This enzyme hydrolyzes glucose-6-phosphate to glucose and phosphate, a critical step in gluconeogenesis and glycogenolysis. Mutations in G6PC1 cause glycogen storage disease type I (von Gierke disease), marked by hypoglycemia and glycogen accumulation. This knockout provides a human hepatic system for studying glucose regulation, metabolic control, and glycogen storage disorders, supporting therapeutic discovery for metabolic and genetic diseases.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	G6PC1
Full Name	Glucose-6-Phosphatase Catalytic Subunit 1
Alternative Name	G6PC; G6PT; GSD1; GSD1a; G6Pase
Location	17q21.31
Gene ID	2538
Summary	Glucose-6-phosphatase (G6Pase) is an endoplasmic reticulum membrane enzyme complex with catalytic and transporter subunits. This gene, G6PC, encodes one of three catalytic subunits and is critical for glucose homeostasis via gluconeogenesis and glycogenolysis. Mutations cause glycogen storage disease type I (von Gierke disease).