GlycoErase™ G6PC3 Knockout CHO-K1 Cell Line (CAT#: GLJF-0825-JF279)

Description	GlycoErase™ G6PC3 knockout CHO-K1 cell line is a CHO-K1 model lacking G6PC3 expression. G6PC3 encodes the catalytic subunit of glucose-6-phosphatase localized in the ER and catalyzes the terminal step of gluconeogenesis and glycogenolysis. Mutations in G6PC3 lead to severe congenital neutropenia, linking glucose metabolism to immune cell survival. This knockout model supports investigations into neutrophil biology, metabolic regulation of immune responses, and disease pathways relevant to congenital neutropenia, while enabling preclinical studies in immunometabolism.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	G6PC3
Full Name	Glucose-6-Phosphatase Catalytic Subunit 3
Alternative Name	SCN4; UGRP
Location	17q21.31
Gene ID	92579
Summary	This gene encodes the catalytic subunit of glucose-6-phosphatase (G6Pase). G6Pase is located in the endoplasmic reticulum (ER) and catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the last step of the gluconeogenic and glycogenolytic pathways. Mutations in this gene result in autosomal recessive severe congenital neutropenia. Alternative splicing results in multiple transcript variants.