GlycoErase™ GBE1 Knockout CHO-K1 Cell Line (CAT#: GLJF-0825-JF282)

Description	GlycoErase™ GBE1 knockout CHO-K1 cell line is a CHO-K1 model in which GBE1 expression has been eliminated. GBE1 encodes the glycogen branching enzyme that generates alpha-1,6 branches in glycogen, essential for solubility and energy balance. Mutations in GBE1 cause glycogen storage disease IV (Andersen's disease). This knockout model provides a tool to study glycogen branching, storage diseases, and cellular osmotic regulation, while serving as a system for therapeutic target validation in glycogen metabolism disorders.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	GBE1
Full Name	1,4-Alpha-Glucan Branching Enzyme 1
Alternative Name	GBE; APBD; GSD4
Location	3p12.2
Gene ID	2632
Summary	The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease).