GlycoErase™ GCG Knockout CHO-K1 Cell Line (CAT#: GLJF-0825-JF283)

Description	GlycoErase™ GCG knockout CHO-K1 cell line is a CHO-K1 model lacking GCG expression. GCG encodes a preproprotein processed into glucagon, which stimulates glycogenolysis and gluconeogenesis, and gut-derived peptides that promote nutrient absorption. The knockout of GCG in CHO-K1 provides a platform for investigating hormone-regulated glucose metabolism, pancreatic hormone signaling, and gut-endocrine cross-talk, with applications in diabetes research, metabolic disease modeling, and hormone-based therapeutics.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	GCG
Full Name	Glucagon
Alternative Name	GLP1; GLP2; GRPP; GLP-1
Location	2q24.2
Gene ID	2641
Summary	The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.