GlycoErase™ GCG Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF631)

Description	GlycoErase™ GCG knockout HepG2 cell line is deficient in GCG, encoding preproglucagon, processed into glucagon and gut-derived peptides. Glucagon stimulates hepatic glycogenolysis and gluconeogenesis, while other peptides regulate nutrient absorption. This knockout provides a human liver model to investigate hormone-driven glucose metabolism, pancreatic-hepatic signaling, and endocrine crosstalk, with applications in diabetes and hormone-based therapies.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	GCG
Full Name	Glucagon
Alternative Name	GLP1; GLP2; GRPP; GLP-1
Location	2q24.2
Gene ID	2641
Summary	The preproprotein encoded by this gene yields four mature peptides, including glucagon, which counteracts insulin by stimulating glycogen breakdown and gluconeogenesis. Other peptides regulate nutrient absorption and resemble glicentin.