GlycoErase™ GCK Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF516)

Description	GlycoErase™ GCK knockout HEK293T cell line is a HEK293T model lacking GCK expression. GCK encodes glucokinase, a glucose sensor in pancreatic β-cells and a regulator of hepatic glucose utilization. Mutations are linked to maturity-onset diabetes of the young (MODY) and hypoglycemia. This knockout provides a human cellular context to study glucose sensing, β-cell signaling mimicry, and therapeutic strategies for glucose homeostasis disorders.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	GCK
Full Name	Glucokinase
Alternative Name	GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; PNDM1; FGQTL3
Location	7p13
Gene ID	2645
Summary	This gene encodes a hexokinase that phosphorylates glucose to glucose-6-phosphate, a key step in glucose metabolism. Unlike other hexokinases, this enzyme is not inhibited by glucose-6-phosphate. It plays a role in insulin secretion in the pancreas and glucose uptake in the liver. Mutations are linked to diabetes and hypoglycemia.