GlycoErase™ GPI Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF635)

Description	GlycoErase™ GPI knockout HepG2 cell line is a human HepG2 model lacking GPI, which encodes glucose phosphate isomerase. This multifunctional "moonlighting" protein acts intracellularly as a glycolytic enzyme and extracellularly as neuroleukin, autocrine motility factor, and angiogenic factor. Deficiency causes hemolytic anemia and neurological impairment. This knockout supports research on glycolysis, tumor-secreted factors, neurotrophic signaling, and immune regulation, with applications in oncology, neurobiology, and metabolic disease.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	GPI
Full Name	Glucose-6-Phosphate Isomerase
Alternative Name	AMF; NLK; PGI; PHI; GNPI; SA36; SA-36; CNSHA4
Location	19q13.11
Gene ID	2821
Summary	This gene encodes glucose-6-phosphate isomerase, a multifunctional protein acting in glycolysis and as an extracellular neurotrophic factor, lymphokine, and tumor-secreted cytokine. Mutations cause nonspherocytic hemolytic anemia and may lead to severe neonatal outcomes.