GlycoErase™ GYS2 Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF525)

Description	GlycoErase™ GYS2 knockout HEK293T cell line is a human model lacking GYS2 expression. GYS2 encodes the liver isoform of glycogen synthase, the rate-limiting enzyme in hepatic glycogen biosynthesis. Loss-of-function mutations cause glycogen storage disease type 0, characterized by fasting hypoglycemia. The knockout in HEK293T provides a system to study hepatic glycogen regulation, energy balance, and childhood metabolic disease in a human cellular setting.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	GYS2
Full Name	Glycogen Synthase 2
Location	12p12.1
Gene ID	2998
Summary	This gene encodes liver glycogen synthase, catalyzing the transfer of glucose to the glycogen molecule during synthesis. Mutations cause glycogen storage disease type 0, characterized by fasting hypoglycemia and low liver glycogen.