GlycoErase™ HEK293F-ΔGFUS Cell Line

This GFUS knockout (HEK293F) cell line lacks the GFUS protein, also known as Tissue Specific Transplantation Antigen P35B, a GDP-L-fucose synthase. This NADP(H)-binding protein catalyzes the two-step epimerase and reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Loss of GFUS function may disrupt GDP-L-fucose synthesis, impacting the formation of various fucosylated glycans and potentially affecting cell signaling, adhesion, and development.

KO Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293F

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

GFUS

GDP-L-fucose synthase

TSTA3, SDR4E1, FX, P35B

8q24.3

NADP(H)-binding protein that catalyzes the conversion of GDP-4-keto-6-D-deoxymannose to GDP-L-fucose, a substrate for fucosyltransferases involved in various glycoconjugate expression.