GlycoErase™ HEK293F-ΔPOMGNT1 Cell Line (CAT#: GLJF-0425-JF329)

Description	This POMGNT1 knockout (HEK293F) cell line lacks the POMGNT1 protein, a protein O-linked mannose O-acetylglucosaminyltransferase 1. This gene encodes a type II transmembrane protein that resides in the Golgi apparatus. It participates in O-mannosyl glycosylation and is specific for alpha-linked terminal mannose. Loss of POMGNT1 function may disrupt O-mannosyl glycosylation.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293F
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	POMGNT1
Full Name	Protein O-linked mannose O-acetylglucosaminyltransferase 1 (Beta 1,2-)
Alternative Name	LGMD2O, FLJ20277, MEB, MGAT1.2
Location	1p34.1
Gene ID	55624
Summary	Golgi-resident type II transmembrane protein involved in O-mannosyl glycosylation, specifically targeting alpha-linked terminal mannose.