GlycoErase™ HEK293T B4GAT1 Knockout Cell Line

To further expand the tools available for studying poly-N-acetyllactosamine synthesis, we are pleased to offer a B4GAT1 knockout HEK293T cell line. This cell line, lacking the B4GAT1-encoded beta-1,4-glucuronyltransferase 1, provides a unique opportunity to investigate the role of this enzyme in the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen. This model, within the context of HEK293T cells, a platform widely used for gene function and regulation studies, allows for in-depth analysis of B4GAT1's contribution to poly-N-acetyllactosamine synthesis and its potential implications for blood group i antigen expression.

KO Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293T

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Penicillin/Streptomycin

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

B4GAT1

Beta-1,4-glucuronyltransferase 1

B3GNT1, iGAT, B3GNT6, B3GN-T1, IGnT, BETA3GNTI

11q13.2

Type II transmembrane protein essential for the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen.