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GlycoErase™ HEK293T ST3GAL1 Knockout Cell Line

CAT#: GLJF-0425-JF470

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Overview Specification Target Information Related Products
Overview
Description
For researchers exploring sialylation, we are pleased to offer an ST3GAL1 knockout HEK293T cell line. This model lacks the ST3GAL1-encoded ST3 beta-galactoside alpha-2,3-sialyltransferase 1, an enzyme that catalyzes the transfer of sialic acid to galactose-containing substrates. By disrupting ST3GAL1 function, this cell line provides a valuable tool for understanding the consequences of impaired sialylation and its impact on cellular processes.
Product Type
KO Cell Lines
Species
Human
Cell Morphology
Epithelial-like, adherent
Passage Ratio
1:2~1:4
Cell Line
HEK293T
Lineage
Embryonic kidney
Specification
Cell Viability
>90%
Sterility Test
The sterility test indicated an absence of microbial growth.
Identity Test
STR identification
Mycoplasma Test
Negative
Virus Test
Negative for HIV, HBV and HCV.
Genetic Stability Testing
We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation
PCR, Sanger Sequencing
Culture Medium
DMEM & FBS & Penicillin/Streptomycin
Application
Functional assay
Size
1 M cells/vial*2
Product Format
Frozen
Shipping
Dry ice
Availability Status
Made to order
Handling Notes
Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer
This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.
Target Information
Target
ST3GAL1
Full Name
ST3 Beta-galactoside alpha-2,3-sialyltransferase 1
Alternative Name
ST3GalA.1, ST3O, SIAT4A, SIATFL
Location
8q24.22
Gene ID
6482
Summary
Catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates; primarily located in the Golgi but can be processed into a soluble form.
For Research Use Only.
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