GlycoErase™ HEK293T ST3GAL1 Knockout Cell Line (CAT#: GLJF-0425-JF470)

Description	For researchers exploring sialylation, we are pleased to offer an ST3GAL1 knockout HEK293T cell line. This model lacks the ST3GAL1-encoded ST3 beta-galactoside alpha-2,3-sialyltransferase 1, an enzyme that catalyzes the transfer of sialic acid to galactose-containing substrates. By disrupting ST3GAL1 function, this cell line provides a valuable tool for understanding the consequences of impaired sialylation and its impact on cellular processes.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ST3GAL1
Full Name	ST3 Beta-galactoside alpha-2,3-sialyltransferase 1
Alternative Name	ST3GalA.1, ST3O, SIAT4A, SIATFL
Location	8q24.22
Gene ID	6482
Summary	Catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates; primarily located in the Golgi but can be processed into a soluble form.