GlycoErase™ HEK293T ST3GAL5 Knockout Cell Line (CAT#: GLJF-0425-JF474)

Description	We are pleased to offer an ST3GAL5 knockout HEK293T cell line for researchers investigating ganglioside GM3 synthesis and its roles in cell differentiation, proliferation, morphology, signal transduction, and adhesion. This cell line lacks the ST3GAL5-encoded ST3 beta-galactoside alpha-2,3-sialyltransferase 5, an enzyme crucial for ganglioside GM3 synthesis. This model provides a valuable tool for elucidating the functions of ganglioside GM3 in various cellular processes.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ST3GAL5
Full Name	ST3 Beta-galactoside alpha-2,3-sialyltransferase 5
Alternative Name	SIAT9, ST3GalV, SIATGM3S
Location	2p11.2
Gene ID	8869
Summary	Catalyzes the formation of ganglioside GM3 from lactosylceramide, playing a role in cell differentiation, proliferation, and signal transduction.