GlycoErase™ LCMT1 Knockout CHO-K1 Cell Line (CAT#: GLJF-0825-JF297)

Description	GlycoErase™ LCMT1 knockout CHO-K1 cell line is a CHO-K1 model lacking LCMT1 expression. LCMT1 catalyzes the methylation of the C-terminal leucine residue of the catalytic subunit of protein phosphatase-2A (PP2A). This modification regulates PP2A activity, a central enzyme in diverse signaling pathways. The knockout model supports studies of phosphatase regulation, signal transduction fidelity, and cancer biology.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	LCMT1
Full Name	Leucine Carboxyl Methyltransferase 1
Alternative Name	LCMT; PPMT1; CGI-68
Location	16p12.1
Gene ID	51451
Summary	LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915).