GlycoErase™ LDHA Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF414)

Description	GlycoErase™ LDHA knockout HEK293 cell line is a HEK293 model in which LDHA expression has been eliminated. LDHA encodes the A subunit of lactate dehydrogenase, catalyzing the interconversion of pyruvate and lactate during anaerobic glycolysis. Mutations are linked to exertional myoglobinuria. This knockout system enables human-focused research on glycolytic flux, lactate accumulation in tumor cells, and metabolic rewiring in cancer and immune responses.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	LDHA
Full Name	Lactate Dehydrogenase A
Alternative Name	LDHM; GSD11; PIG19; HEL-S-133P
Location	11p15.1
Gene ID	3939
Summary	This gene encodes the A subunit of lactate dehydrogenase, an enzyme catalyzing the conversion of pyruvate to lactate. It is mainly expressed in skeletal muscle and mutations lead to exertional myoglobinuria. Pseudogenes of this gene exist.