GlycoErase™ PCK1 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF422)

Description	GlycoErase™ PCK1 knockout HEK293 cell line is a human embryonic kidney (HEK293) model without PCK1 expression. PCK1 encodes cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C), a rate-controlling enzyme in gluconeogenesis converting oxaloacetate to phosphoenolpyruvate. Mutations cause PEPCK deficiency. This knockout model supports research on hepatic glucose metabolism, diabetes mechanisms, and endocrine regulation in a human cellular background.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PCK1
Full Name	Phosphoenolpyruvate Carboxykinase 1
Alternative Name	PCKDC; PEPCK1; PEPCKC; PEPCK-C
Location	20q13.31
Gene ID	5105
Summary	This gene regulates gluconeogenesis by catalyzing the formation of phosphoenolpyruvate from oxaloacetate. It is regulated by insulin, glucocorticoids, and other factors. Defects lead to cytosolic phosphoenolpyruvate carboxykinase deficiency.