GlycoErase™ PGK1 Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF547)

Description	GlycoErase™ PGK1 knockout HEK293T cell line is a human embryonic kidney model lacking PGK1 expression. PGK1 encodes phosphoglycerate kinase 1, which converts 1,3-bisphosphoglycerate to 3-phosphoglycerate in glycolysis, and also moonlights as an angiogenic and tumor-promoting factor. Its knockout enables study of glycolysis-angiogenesis crosstalk, tumor biology, and metabolic disease.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PGK1
Full Name	Phosphoglycerate Kinase 1
Alternative Name	PGKA; MIG10; HEL-S-68p
Location	Xq21.1
Gene ID	5230
Summary	This gene encodes a glycolytic enzyme that converts 1,3-diphosphoglycerate to 3-phosphoglycerate. It also plays a role in angiogenesis by reducing disulfide bonds in plasmin. Deficiency is linked to hemolytic anemia and neurological impairment.