GlycoErase™ PHKA2 Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF552)

Description	GlycoErase™ PHKA2 knockout HEK293T cell line removes PHKA2 expression. PHKA2 encodes the liver isoform of the phosphorylase kinase alpha subunit, and mutations result in glycogen storage disease type IXA. This model is suitable for examining liver-specific glycogen metabolism, energy control, and metabolic liver disease.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PHKA2
Full Name	Phosphorylase Kinase Regulatory Subunit Alpha 2
Alternative Name	PHK; PYK; XLG; PYKL; XLG2; GSD9A
Location	Xp22.13
Gene ID	5256
Summary	Phosphorylase kinase is a 16-subunit enzyme containing four alpha, beta, gamma, and delta units. The hepatic alpha isoform is encoded by this gene, while the gamma unit carries the active site and the delta subunit (calmodulin) regulates calcium sensitivity. Mutations in this gene result in glycogen storage disease type 9A (X-linked liver glycogenosis). Alternative transcripts have been observed but not fully characterized.