GlycoErase™ PHKB Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF553)

Description	GlycoErase™ PHKB knockout HEK293T cell line disrupts PHKB expression. PHKB encodes the beta regulatory subunit of phosphorylase kinase, expressed in both liver and muscle isoforms. Mutations lead to glycogen storage disease type IXB. This knockout provides a platform for exploring regulatory mechanisms of glycogen breakdown, multisystem metabolic defects, and calcium-dependent signaling.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PHKB
Full Name	Phosphorylase Kinase Regulatory Subunit Beta
Location	16q12.1
Gene ID	5257
Summary	This enzyme complex is composed of 16 subunits: alpha, beta, gamma, and delta. The beta subunit, encoded by this gene, is identical in both liver and muscle isoforms. The gamma unit contains the catalytic domain, and the delta unit regulates calcium dependence. Variants in this gene cause glycogen storage disease type 9B. Pseudogenes are located on chromosomes 14 and 20.