GlycoErase™ PHKG1 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF438)

Description	GlycoErase™ PHKG1 knockout HEK293 cell line is a HEK293 model lacking PHKG1 expression. PHKG1 encodes the skeletal muscle gamma subunit of phosphorylase kinase, which contains both catalytic and calmodulin-binding domains critical for activity. This knockout provides a system for analyzing calcium-dependent regulation of glycogen breakdown, kinase function, and potential therapeutic strategies for muscle energy disorders.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PHKG1
Full Name	Phosphorylase Kinase Catalytic Subunit Gamma 1
Alternative Name	PHKG
Location	7p11.2
Gene ID	5260
Summary	This gene encodes a Ser/Thr protein kinase with one kinase domain and two calmodulin-binding domains. It is the catalytic subunit of a 16-subunit phosphorylase kinase complex essential for glycogen breakdown. Pseudogenes exist on chromosomes 7q11.21 and 11p11.12. Multiple transcript variants have been identified.