GlycoErase™ PPP1R12A Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF444)

Description	GlycoErase™ PPP1R12A knockout HEK293 cell line is a HEK293 model without PPP1R12A (MYPT1). This regulatory subunit modulates myosin phosphatase activity downstream of RhoA-Rho-kinase signaling. The knockout cells serve as human-derived models to investigate cytoskeletal remodeling, adhesion signaling, and cell motility relevant to cancer metastasis and fibrosis research.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1R12A
Full Name	Protein Phosphatase 1 Regulatory Subunit 12A
Alternative Name	MBS; GUBS; M130; MYPT1
Location	12q21.2-q21.31
Gene ID	4659
Summary	Myosin phosphatase target subunit 1 (MYPT1) is a regulatory subunit of myosin phosphatase, which regulates actin-myosin interactions. MYPT1 is involved in smooth muscle contraction and non-muscle cell functions, with several isoforms identified.