GlycoErase™ PPP1R16A Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF451)

Description	GlycoErase™ PPP1R16A knockout HEK293 cell line is a HEK293 model without PPP1R16A, a MYPT subunit that targets PP1c to myosin, with activation uniquely regulated by PKA. This knockout supports research into noncanonical PP1 regulation, motility signaling, and PKA-dependent cytoskeletal dynamics in human cells.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1R16A
Full Name	Protein Phosphatase 1 Regulatory Subunit 16A
Alternative Name	MYPT3
Location	8q24.3
Gene ID	84988
Summary	This MYPT subunit directs PP1c to myosin light chain targets, activating PP1c upon phosphorylation by protein kinase A, unlike other MYPT members whose phosphorylation inhibits PP1c.