GlycoErase™ PPP1R16A Knockout Jurkat Cell Line (CAT#: GLJF-0825-JF799)

Description	GlycoErase™ PPP1R16A knockout Jurkat cell line is a Jurkat T cell model without PPP1R16A, a MYPT subunit directing PP1c to myosin targets. Unlike other MYPT members, phosphorylation by PKA activates PP1c. Knockout systems allow analysis of noncanonical PP1 regulation and cell motility in T cells, offering insights into T cell migration and adhesion in immune responses.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Suspension-growing
Passage Ratio	1:3~1:5
Cell Line	Jurkat cell line
Primary Disease	T-cell acute lymphoblastic leukemia (T-ALL)
Lineage	T Lymphocyte
Lineage Subtype	T-cell acute lymphoblastic leukemia (T-ALL)

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1R16A
Full Name	Protein Phosphatase 1 Regulatory Subunit 16A
Alternative Name	MYPT3
Location	8q24.3
Gene ID	84988
Summary	Myosin light chain kinase and phosphatase (MLCP) complexes control the phosphorylation states of regulatory myosin light chains, which is crucial for muscle and intracellular movement. MLCPs typically contain a catalytic protein phosphatase 1 (PP1c) subunit, a myosin phosphatase targeting (MYPT) subunit, and another smaller subunit. The protein encoded by this gene represents an MYPT subunit, which is responsible for directing PP1c to its intended targets. However, while the phosphorylation of other MYPT members results in PP1c inactivation, phosphorylation of the encoded protein by protein kinase A results in PP1c activation.