GlycoErase™ PPP1R8 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF465)

Description	GlycoErase™ PPP1R8 knockout HEK293 cell line is a HEK293 model in which PPP1R8 expression has been removed. PPP1R8 encodes isoforms that either inhibit PP1 or act as a magnesium-dependent endoribonuclease that cleaves A+U-rich RNAs. The knockout provides a versatile human system for dissecting phosphatase inhibition versus RNA-processing functions, with applications in stress signaling, RNA stability, and cancer-related phosphatase-RNA interactions.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1R8
Full Name	Protein Phosphatase 1 Regulatory Subunit 8
Alternative Name	ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
Location	1p35.3
Gene ID	5511
Summary	Through alternative splicing, this gene encodes three isoforms: two that inhibit type 1 serine/threonine phosphatases and one that is a single-strand endoribonuclease similar to bacterial RNase E, which cleaves A+U-rich RNA regions in a magnesium-dependent manner.