GlycoErase™ PRKAB2 Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF589)

Description	GlycoErase™ PRKAB2 knockout HEK293T cell line is a human HEK293T model lacking PRKAB2, a regulatory β subunit of AMPK highly expressed in skeletal muscle. This subunit modulates AMPK activation during metabolic stress and regulates fatty acid and cholesterol biosynthesis. The knockout provides a tool for dissecting AMPK tissue-specific functions, metabolic stress adaptation, and energy-sensing pathways related to muscle physiology.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PRKAB2
Full Name	Protein Kinase Amp-Activated Non-Catalytic Subunit Beta 2
Location	1q21.1
Gene ID	5565
Summary	This beta regulatory subunit of AMPK is predominantly expressed in skeletal muscle and may have muscle-specific metabolic roles. It positively modulates AMPK activity and is expressed in multiple isoforms.