GlycoErase™ PRKACA Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF474)

Description	GlycoErase™ PRKACA knockout HEK293 cell line is a HEK293 model with PRKACA deleted. PRKACA encodes a catalytic subunit of protein kinase A (PKA), a tetrameric holoenzyme regulated by cAMP. Dissociation of regulatory and catalytic subunits activates PKA, enabling phosphorylation of proteins involved in proliferation, differentiation, and apoptosis. Dysregulation has been linked to adrenal hyperplasias and Cushing's syndrome. This knockout system offers a human context for dissecting PKA signaling, cAMP-driven processes, and endocrine-related oncogenesis.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PRKACA
Full Name	Protein Kinase Camp-Activated Catalytic Subunit Alpha
Alternative Name	CAFD1; PKACA; PPNAD4
Location	19p13.12
Gene ID	5566
Summary	This gene encodes one of the catalytic subunits of protein kinase A (PKA). Inactive PKA is a tetramer of two regulatory and two catalytic subunits; cAMP binding releases active catalytic monomers. Mutations and duplications involving this gene are linked to adrenal hyperplasia and Cushing's syndrome.