GlycoErase™ PRKAG2 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF476)

Description	GlycoErase™ PRKAG2 knockout HEK293 cell line is a HEK293 model with PRKAG2 expression eliminated. PRKAG2 encodes a regulatory γ-subunit of AMPK that modulates enzyme activity by binding adenine nucleotides. Mutations are associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage diseases of the heart. This knockout provides a human background to study AMPK regulation, cardiometabolic pathways, and disease-linked energy metabolism.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PRKAG2
Full Name	Protein Kinase Amp-Activated Non-Catalytic Subunit Gamma 2
Alternative Name	AAKG; CMH6; WPWS; AAKG2; H91620p
Location	7q36.1
Gene ID	51422
Summary	This gene encodes the gamma subunit of AMP-activated protein kinase (AMPK), an energy sensor regulating lipid and cholesterol biosynthesis. Mutations are associated with Wolff-Parkinson-White syndrome, hypertrophic cardiomyopathy, and cardiac glycogen storage disease. Multiple isoforms exist.