GlycoErase™ SLC35D1 Knockout CHO-K1 Cell Line (CAT#: GLJF-0425-JF84)

Description	The SLC35D1 knockout CHO-K1 cell line, constructed using gene editing technology to knockout solute carrier family 35 member D1, research indicates that this cell line may fundamentally disrupt the efficient transport of crucial nucleotide sugars like UDP-GlcA and UDP-GalNAc into the ER lumen of CHO cells, as SLC35D1 protein specifically responsible for transporting multiple cytosolic nucleotide sugars to ER, this will directly and profoundly affect the core function of glycosylation pathways for initial protein glycosylation in CHO cell endoplasmic reticulum.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SLC35D1
Full Name	Solute carrier family 35 member D1
Alternative Name	KIAA0260, UGTREL7
Location	1p31.3
Gene ID	23169
Summary	Transports UDP-glucuronic acid and UDP-N-acetylgalactosamine from the cytoplasm to the endoplasmic reticulum lumen, essential for glycosylation of cellular glycoconjugates.