GlycoErase™ SLC35D2 Knockout CHO-K1 Cell Line

The SLC35D2 knockout CHO-K1 cell line, successfully prepared without solute carrier family 35 member D2, this cell line may fundamentally interfere with the precise transport process of nucleotide sugars into the Golgi lumen of CHO cells, given that SLC35D2 protein directly participates in supplying multiple substrates for Golgi glycosylation reactions, knockout of SLC35D2 is expected to broadly and significantly impact Golgi glycosylation reaction efficiency and final product quality for high-efficiency biopharmaceutical manufacturing in CHO cells.

KO Cell Lines

Hamster

Epithelial, adherent

1:3~1:5

CHO-K1

Chinese hamster ovary

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & Proline & RPMI

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

SLC35D2

Solute carrier family 35 member D2

SQV7L, UGTrel8

Functions as a nucleotide sugar transporter, specifically transporting UDP-N-acetylglucosamine into the Golgi apparatus for use in glycosylation reactions.