GlycoErase™ ST3GAL4 Knockout CHO-K1 Cell Line (CAT#: GLJF-0425-JF89)

Description	The ST3GAL4 knockout CHO-K1 cell line, we have obtained a CHO-K1 cell line with gene knockout of ST3 beta-galactoside alpha-2,3-sialyltransferase 4, preliminary analysis suggests that this cell line may have a certain impact on the overall sialylation modification level of CHO cells, ST3GAL4 protein belongs to the functionally diverse glycosyltransferase 29 family, the cell model constructed with its functional deficiency will help in-depth analysis of the precise function and mechanism of action of this enzyme family in the complex glycosylation modification network of CHO cells.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ST3GAL4
Full Name	ST3 Beta-galactoside alpha-2,3-sialyltransferase 4
Alternative Name	SIAT4, STZ, NANTA3, SIAT4C, SAT3, CGS23, FLJ11867
Location	11q24.2
Gene ID	6484
Summary	Golgi-localized glycosyltransferase involved in protein glycosylation; may be secreted and potentially contributes to the regulation of sialyl Lewis X antigen expression during inflammation.