GlycoErase™ VIP Knockout CHO-K1 Cell Line (CAT#: GLJF-0825-JF373)

Description	GlycoErase™ VIP knockout CHO-K1 cell line is a CHO-K1 model in which VIP expression has been eliminated. VIP encodes vasoactive intestinal peptide, a glucagon-family neuropeptide that promotes vasodilation, glycogenolysis, smooth muscle relaxation, and antimicrobial activity through cAMP-dependent signaling. The knockout offers a defined platform for interrogating neuropeptide signaling, prostanoid/GPCR crosstalk, and metabolic and vascular regulation, supporting applications in inflammation, cardiovascular biology, and metabolism.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	VIP
Full Name	Vasoactive Intestinal Peptide
Alternative Name	PHM27
Location	6q25.2
Gene ID	7432
Summary	The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.