GlycoFlux™ AAV1-Human PHKB Viral Particle

GlycoFlux™ AAV1-PHKB Viral Particle delivers persistent PHKB (β subunit) expression shared by liver and muscle isoforms to tune holoenzyme regulation and investigate GSD-IXB; this product transduces pancreas and smooth muscle for long-term glycogen-signal integration studies in a replication-defective, research-only format.

Recombinant AAV1

PHKB

Human

CMV, CAG, TRE, or other promoters

Smooth muscle, Central Nervous System (CNS), Retina, Pancreas, Inner Ear, Heart, Liver

AAV Triple-Plasmid Transfection System

HEK293T

This AAV1 viral particle is primarily used for overexpressing target genes in muscle tissues (smooth muscle, heart) and the central nervous system (CNS) / sensory organs (retina, inner ear). It is an ideal tool for research and pre-clinical studies focused on myopathies, cardiac disorders, and certain neurological or sensory genetic diseases.

Shipped on dry ice, with temperature maintained at ≤ -80°C.

Store at -80°C to preserve viral titer and stability.

Store this product at -80°C and minimize freeze-thaw cycles to preserve viral titer. For safety, handle in a certified biological safety cabinet and wear appropriate PPE (lab coat, gloves, and eye protection).

This product is intended for research use only and is not approved for diagnostic procedures or clinical applications. Users are solely responsible for implementing appropriate safety practices and complying with all applicable laws, regulations, and institutional policies. While Creative Biolabs is committed to rigorous quality control, no guarantee is made regarding performance in any particular assay or application.

qPCR/TCID50

No mycoplasma contamination was detected.

Sterility testing verified the absence of microbial contamination.

Upon request, Creative Biolabs performs in vitro or in vivo transduction assays to assess AAV delivery to target cells and to quantify transgene expression and function.

All AAV preparations from Creative Biolabs are verified for vector-genome identity by PCR. In this assay, target cells are transduced with serial dilutions of the AAV preparation, harvested after a short incubation, and genomic DNA is isolated; a defined region of the packaged transgene is then PCR-amplified to confirm the expected sequence.

Phosphorylase Kinase Regulatory Subunit Beta

16q12.1

Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.