Description

GlycoFlux™ AAV1-PRKACA Viral Particle provides durable PRKACA expression to release active PKA catalytic monomers upon cAMP elevation and interrogate cAMP-dependent phosphorylation in differentiation, proliferation, and apoptosis, and this product demonstrates strong delivery to central nervous system, liver, and retina for prolonged signal-transduction and endocrine-pathway modeling with replication-incompetent handling.

Product Type

Recombinant AAV1

Target

PRKACA

Species

Human

Promotor

CMV, CAG, TRE, or other promoters

Tissue Tropism

Smooth muscle, Central Nervous System (CNS), Retina, Pancreas, Inner Ear, Heart, Liver

Packaging System

AAV Triple-Plasmid Transfection System

Packaging Cell

HEK293T

Application

This AAV1 viral particle is primarily used for overexpressing target genes in muscle tissues (smooth muscle, heart) and the central nervous system (CNS) / sensory organs (retina, inner ear). It is an ideal tool for research and pre-clinical studies focused on myopathies, cardiac disorders, and certain neurological or sensory genetic diseases.

Shipping

Shipped on dry ice, with temperature maintained at ≤ -80°C.

Storage

Store at -80°C to preserve viral titer and stability.

Handling Notes

Store this product at -80°C and minimize freeze-thaw cycles to preserve viral titer. For safety, handle in a certified biological safety cabinet and wear appropriate PPE (lab coat, gloves, and eye protection).

Product Disclaimer

This product is intended for research use only and is not approved for diagnostic procedures or clinical applications. Users are solely responsible for implementing appropriate safety practices and complying with all applicable laws, regulations, and institutional policies. While Creative Biolabs is committed to rigorous quality control, no guarantee is made regarding performance in any particular assay or application.

Titer Assay	qPCR/TCID50
Mycoplasma Test	No mycoplasma contamination was detected.
Sterility	Sterility testing verified the absence of microbial contamination.
Transduction Evaluation	Upon request, Creative Biolabs performs in vitro or in vivo transduction assays to assess AAV delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	All AAV preparations from Creative Biolabs are verified for vector-genome identity by PCR. In this assay, target cells are transduced with serial dilutions of the AAV preparation, harvested after a short incubation, and genomic DNA is isolated; a defined region of the packaged transgene is then PCR-amplified to confirm the expected sequence.

Full Name	Protein Kinase Camp-Activated Catalytic Subunit Alpha
Alternative Name	CAFD1; PKACA; PPNAD4
Location	19p13.12
Gene ID	5566
Summary	This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

GlycoFlux™ AAV1-Human PRKACA Viral Particle (CAT#: GLJF-1025-JF1013)

Specifications

Quality Control

Target Information

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