Specifications Quality Control Target Information Related Products

Specifications

Description

GlycoFlux™ AAV1-ST3GAL1 Viral Particle provides stable ST3GAL1 expression to transfer sialic acid from CMP-Neu5Ac to Gal-containing acceptors, and this product exhibits preferred transduction in heart and retina to support long-term control of O-glycan sialylation and antigen display.

Product Type

Recombinant AAV1

Target

ST3GAL1

Species

Human

Promotor

CMV, CAG, TRE, or other promoters

Tissue Tropism

Smooth muscle, Central Nervous System (CNS), Retina, Pancreas, Inner Ear, Heart, Liver

Packaging System

AAV Triple-Plasmid Transfection System

Packaging Cell

HEK293T

Application

This AAV1 viral particle is primarily used for overexpressing target genes in muscle tissues (smooth muscle, heart) and the central nervous system (CNS) / sensory organs (retina, inner ear). It is an ideal tool for research and pre-clinical studies focused on myopathies, cardiac disorders, and certain neurological or sensory genetic diseases.

Shipping

Shipped on dry ice, with temperature maintained at ≤ -80°C.

Storage

Store at -80°C to preserve viral titer and stability.

Handling Notes

Store this product at -80°C and minimize freeze-thaw cycles to preserve viral titer. For safety, handle in a certified biological safety cabinet and wear appropriate PPE (lab coat, gloves, and eye protection).

Product Disclaimer

This product is intended for research use only and is not approved for diagnostic procedures or clinical applications. Users are solely responsible for implementing appropriate safety practices and complying with all applicable laws, regulations, and institutional policies. While Creative Biolabs is committed to rigorous quality control, no guarantee is made regarding performance in any particular assay or application.

Quality Control

Titer Assay

qPCR/TCID50

Mycoplasma Test

No mycoplasma contamination was detected.

Sterility

Sterility testing verified the absence of microbial contamination.

Transduction Evaluation

Upon request, Creative Biolabs performs in vitro or in vivo transduction assays to assess AAV delivery to target cells and to quantify transgene expression and function.

Insert Identity Confirmation

All AAV preparations from Creative Biolabs are verified for vector-genome identity by PCR. In this assay, target cells are transduced with serial dilutions of the AAV preparation, harvested after a short incubation, and genomic DNA is isolated; a defined region of the packaged transgene is then PCR-amplified to confirm the expected sequence.

Target Information

Full Name

ST3 Beta-galactoside alpha-2,3-sialyltransferase 1

Alternative Name

ST3GalA.1; ST3O; SIAT4A; SIATFL

Location

8q24.22

Gene ID

6482

Summary

Catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates; primarily located in the Golgi but can be processed into a soluble form.

GlycoFlux™ Lenti-Human ST3GAL1 Viral Particle (CAT#: GLJF-1025-JF118)
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GlycoFlux™ AAV1-Human ST3GAL1 Viral Particle