GlycoFlux™ Human ACLY Overexpression Glycoengineered Cell (CAT#: GLJF-0925-JF1)

Description	GlycoFlux™ human ACLY overexpression glycoengineered cell is a stable cell line that constitutively overexpresses ATP-citrate lyase (ACLY), the primary source of cytosolic acetyl-CoA in many tissues. ACLY is a ~440-kDa homotetramer that converts citrate and CoA to acetyl-CoA and oxaloacetate with concomitant ATP hydrolysis (ADP + Pi). The resulting acetyl-CoA fuels lipogenesis and cholesterogenesis, and ACLY activity in nervous tissue may contribute to acetylcholine biosynthesis. This model elevates acetyl-CoA flux for studies of lipid/cholesterol metabolism, acetylation-dependent pathways, and screening/validation of ACLY modulators; multiple ACLY transcript variants/isoforms can be profiled as needed.
Product Type	Overexpression Cell Lines
Cell Line	As requested by the client.

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ACLY
Full Name	Atp Citrate Lyase
Alternative Name	ACL; ATPCL; CLATP
Location	17q21.2
Gene ID	47
Summary	ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.