GlycoFlux™ Human ACLY Overexpression Glycoengineered Cell

GlycoFlux™ human ACLY overexpression glycoengineered cell is a stable cell line that constitutively overexpresses ATP-citrate lyase (ACLY), the primary source of cytosolic acetyl-CoA in many tissues. ACLY is a ~440-kDa homotetramer that converts citrate and CoA to acetyl-CoA and oxaloacetate with concomitant ATP hydrolysis (ADP + Pi). The resulting acetyl-CoA fuels lipogenesis and cholesterogenesis, and ACLY activity in nervous tissue may contribute to acetylcholine biosynthesis. This model elevates acetyl-CoA flux for studies of lipid/cholesterol metabolism, acetylation-dependent pathways, and screening/validation of ACLY modulators; multiple ACLY transcript variants/isoforms can be profiled as needed.

Overexpression Cell Lines

As requested by the client.

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

qPCR, Sanger Sequencing

Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ACLY

Atp Citrate Lyase

ACL; ATPCL; CLATP

17q21.2

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.