GlycoFlux™ Human ALG10 Overexpression Raji Cell Line (CAT#: GLJF-0925-JF1162)

Description	GlycoFlux™ human ALG10 overexpression Raji cell line is a stable cell line engineered to overexpress ALG10, a glycosyltransferase that catalyzes the addition of the third glucose to the dolichol-linked oligosaccharide precursor inN-linked glycosylation. This model supports studies of protein folding, ER quality control, and congenital glycosylation disorders.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Lymphoblast-like, suspension growth
Passage Ratio	1:2~1:4
Cell Line	Raji
Primary Disease	Burkitt's lymphoma
Lineage	B lymphocyte (B cell)
Lineage Subtype	Mature B cell, widely used in EBV-related studies

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & RPMI
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ALG10
Full Name	ALG10 alpha-1,2-glucosyltransferase
Alternative Name	ALG10A; DIE2; FLJ14751
Location	12p11.1
Gene ID	84920
Summary	This gene encodes a membrane-associated protein that plays a critical role in N-linked glycosylation by adding the third glucose residue to the lipid-linked oligosaccharide precursor. Specifically, it transfers the terminal glucose molecule from dolichyl phosphate glucose (Dol-P-Glc) to the lipid-linked oligosaccharide Glc₂Man₉GlcNAc₂-PP-Dol. This step is essential for the proper assembly of the oligosaccharide precursor necessary for N-linked glycoprotein synthesis.