GlycoFlux™ Human ENO3 Overexpression Glycoengineered Cell (CAT#: GLJF-0925-JF14)

Description	GlycoFlux™ human ENO3 overexpression glycoengineered cell is a stable cell line overexpressing β-enolase, the muscle-enriched isozyme. Use it to dissect isozyme-specific effects on nucleotide-sugar pools, myogenic differentiation glycome trajectories (adhesion receptor glycosylation, laminin-binding), and glycan-dependent stability of secreted reporters under high-demand ATP regimes characteristic of skeletal muscle metabolism.
Product Type	Overexpression Cell Lines
Cell Line	As requested by the client.

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ENO3
Full Name	Enolase 3
Alternative Name	MSE; GSD13
Location	17p13.2
Gene ID	2027
Summary	This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.