GlycoFlux™ Human GCK Overexpression HEK293 Cell Line (CAT#: GLJF-0925-JF400)

Description	GlycoFlux™ human GCK overexpression HEK293 cell line is a stable glycoengineered line that constitutively overexpresses glucokinase (GCK), a low-affinity hexokinase that converts glucose to glucose-6-phosphate without feedback inhibition by G6P. Elevated GCK shifts carbon into glycolysis, the pentose-phosphate and hexosamine pathways, enabling controlled modulation of UDP-GlcNAc/UDP-GalNAc donor pools. Use this model to link nutrient sensing to N-/O-glycan branching, sialylation, and glycoprotein secretion quality, and to benchmark small-molecule GCK modulators for glyco-phenotype outcomes.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & DMEM
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	GCK
Full Name	Glucokinase
Alternative Name	GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; PNDM1; FGQTL3
Location	7p13
Gene ID	2645
Summary	This gene encodes a hexokinase that phosphorylates glucose to glucose-6-phosphate, initiating glucose metabolism. Unlike other hexokinases, this enzyme is not inhibited by its product, glucose-6-phosphate. It is expressed in the pancreas and liver, playing key roles in insulin secretion and glucose uptake. Mutations are associated with diabetes and hyperinsulinemic hypoglycemia.