GlycoFlux™ Human GPI Overexpression HEK293 Cell Line (CAT#: GLJF-0925-JF403)

Description	GlycoFlux™ human GPI overexpression HEK293 cell line is a stable glycoengineered line that constitutively overexpresses glucose-6-phosphate isomerase (GPI), a moonlighting protein (neuroleukin/AMF) interconverting G6P and F6P. By rewiring glycolytic flux and hexosamine precursor balance, it allows causal testing of how cytosolic metabolism shapes N-glycan branching, surface glycoprotein display, and cytokine-like GPI export/biology.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & DMEM
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	GPI
Full Name	Glucose-6-Phosphate Isomerase
Alternative Name	AMF; NLK; PGI; PHI; GNPI; SA36; SA-36; CNSHA4
Location	19q13.11
Gene ID	2821
Summary	This gene encodes a member of the glucose phosphate isomerase family. It functions both as a glycolytic enzyme and a neurotrophic factor (neuroleukin) for motor neurons. Additionally, it serves as a cytokine (autocrine motility factor) promoting tumor cell motility and angiogenesis. Defects lead to nonspherocytic hemolytic anemia, and severe deficiency is linked to hydrops fetalis and neurological issues.