GlycoFlux™ Human GYS1 Overexpression HepG2 Cell Line (CAT#: GLJF-0925-JF640)

Description	GlycoFlux™ human GYS1 overexpression HepG2 cell line is a stable cell line engineered to express glycogen synthase 1 (GYS1), the muscle isoform that elongates glycogen chains through α-1,4-glycosidic linkage formation. Mutations in GYS1 are associated with muscle glycogen storage disease and exercise intolerance. This model provides a platform for studying glycogen biosynthesis, muscle energy metabolism, and therapeutic strategies targeting glycogen storage disorders.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	GYS1
Full Name	Glycogen Synthase 1
Alternative Name	GSY; GYS
Location	19q13.33
Gene ID	2997
Summary	This gene encodes an enzyme that elongates glycogen chains by forming alpha-1,4-glycosidic linkages. Mutations are linked to muscle glycogen storage disease.